Anti-Myostatin Propeptide antibody [4B9] (ab37257)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number4B9
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P 1/200.
IHC-Fr 1/50.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionActs specifically as a negative regulator of skeletal muscle growth.
  • Involvement in diseaseDefects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cmpt antibody
    • GDF-8 antibody
    • GDF8_HUMAN antibody
    • Growth/differentiation factor 8 antibody
    • MSLHP antibody
    • MSTN antibody
    • MSTN myostatin antibody
    • Myostatin antibody
    • Myostatin Propeptide antibody
    see all

Anti-Myostatin Propeptide antibody [4B9] images

  • ICC/IF image of ab37257 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37257, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Myostatin Propeptide antibody [4B9] (ab37257)

ab37257 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"