Recombinant protein, corresponding to amino acids 24-266 of Human Myostatin Propeptide (Human myostatin propeptide without signaling sequence). The recombinant protein contains 5 additional amino acid residues: 2 at the N-terminal end (MG) and 2 at the C-termial end (KLN).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
Use a concentration of 10 µg/ml.
FunctionActs specifically as a negative regulator of skeletal muscle growth.
Involvement in diseaseDefects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.
Sequence similaritiesBelongs to the TGF-beta family.
ICC/IF image of ab37257 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37257, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Myostatin Propeptide antibody [4B9] (ab37257)
has not yet been referenced specifically in any publications.
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