Anti-Myostatin Propeptide antibody [6E8] (ab37254)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number6E8
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
IHC-P 1/100.

Target

  • FunctionActs specifically as a negative regulator of skeletal muscle growth.
  • Involvement in diseaseDefects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cmpt antibody
    • GDF-8 antibody
    • GDF8_HUMAN antibody
    • Growth/differentiation factor 8 antibody
    • MSLHP antibody
    • MSTN antibody
    • MSTN myostatin antibody
    • Myostatin antibody
    • Myostatin Propeptide antibody
    see all

Anti-Myostatin Propeptide antibody [6E8] images

  • IHC image of ab37254 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37254, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Myostatin Propeptide antibody [6E8] (ab37254)

ab37254 has not yet been referenced specifically in any publications.

Product Wall

I have performed a homology search of the first 270 amino acids of the human myostatin precursor with the cow protein and found a homology of 92%. As the antibody is monoclonal it will only recognise one epitope (unlike a polyclonal) and as this has ...

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Thank you for your enquiry regarding ab37254. This antibody was made in tissue culture. Where ascites has been used this will be detailed on the datasheet. Protein G purification is a simple method to select all immunoglobulins (non specifically...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"