Overview

  • Product name
  • Description
    Rabbit polyclonal to MYPN
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 972-1021 (PVPKVYWFKD GKQISKRNEH CKMRREGDGT CSLHIESTTS DDDGNYTIMA) of Human MYPN (NP_115967).

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 2% Sucrose, 97% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.
  • Tissue specificity
    Expressed in adult skeletal muscle and fetal heart.
  • Sequence similarities
    Belongs to the myotilin/palladin family.
    Contains 5 Ig-like (immunoglobulin-like) domains.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Bound to sarcomere both at the Z-line periphery and in the central I-band region.
  • Information by UniProt
  • Database links
  • Alternative names
    • 145 kDa sarcomeric protein antibody
    • MYOP antibody
    • Myopalladin antibody
    • MYPN antibody
    • MYPN_HUMAN antibody
    • sarcomeric protein myopalladin antibody
    see all

Anti-MYPN antibody images

  • Anti-MYPN antibody (ab115775) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 145 kDa

References for Anti-MYPN antibody (ab115775)

ab115775 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart left ventricle and tibialis anterior muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
Heart left ventricle and tibialis anterior muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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