Overview

  • Product name
  • Description
    Goat polyclonal to MYRIP
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: KDLMEPALESAVMY, corresponding to C terminal amino acids 846-859 of Human MYRIP.

  • General notes


    A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa.Can be blocked with Human MYRIP peptide (ab23187).
IP Use at an assay dependent dilution. PubMed: 19706440
ICC/IF Use at an assay dependent dilution. PubMed: 19270261

Target

  • Function
    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments.
  • Tissue specificity
    Detected in brain, skin, heart, adrenal medulla, pancreas, intestine, liver, kidney, muscle and testis.
  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • Cellular localization
    Cytoplasm. In pre- and post-synaptic areas in photoreceptor cells and in the basal microvilli of retinal pigment epithelium cells. Associated with melanosomes. Colocalizes with actin filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586F1018 antibody
    • Exophilin 8 antibody
    • Exophilin antibody
    • Exophilin-8 antibody
    • Exophilin8 antibody
    • FLJ44025 antibody
    • MGC130034 antibody
    • MGC130035 antibody
    • Myosin VIIA and Rab interacting protein antibody
    • Myosin-VIIa- and Rab-interacting protein antibody
    • Myrip antibody
    • MYRIP_HUMAN antibody
    • Rab effector MyRIP antibody
    • SLAC 2C antibody
    • SLAC2 C antibody
    • SlaC2-c antibody
    • SLAC2C antibody
    • Slp homolog lacking C2 domains c antibody
    • Slp homologue lacking C2 domains antibody
    • Slp homologue lacking C2 domains c antibody
    • Synaptotagmin like protein homologue lacking C2 domains c antibody
    • synaptotagmin-like protein lacking C2 domains C antibody
    see all

Images

  • Anti-MYRIP antibody (ab10149) at 0.3 µg/ml + Human Amygdala lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 96 kDa

References

This product has been referenced in:
  • Conte IL  et al. Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis. J Cell Sci 129:592-603 (2016). Read more (PubMed: 26675235) »
  • Song J  et al. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A 106:14966-71 (2009). IP ; Human . Read more (PubMed: 19706440) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Non-Small Cell Lung cell line)
Loading amount
25 µg
Specification
Non-Small Cell Lung cell line
Treatment
siRNA to MYRIP for 72h
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Mar 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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