Anti-n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)

Hi,

This antibody for n-Myc, does it discriminate between the different MYC family members (MYC and MYCL1) in human cells? IE, does it detect n-Myc and not detect c-Myc or Mycl?

Thanks for your help,

ANSWER:

Thank you for your enquiry.

According to the following publication, monoclonal ab16898 n-Myc antibody [clone NCM II 100] interacts with an epitope unique to an N-myc region not present in C-myc regions tested (3' 60% mouse C-myc coding region and 5' 30% C-myc coding region); please see the second paragraph on page 5930 of the publication.

I hope this is helpful. Please do not hesitate to contact me if you require any further assistance.

I would like to find an antibody that is specific of the N-terminal part of the MYCN protein and which is suitable for IP. I would be interested in purchasing the ab16898 MYCN antibody (NCM II 100) but I would like first to know which part of the MYCN protein does this antibody recognize (N-Term, C-Term?).

ANSWER:

Thank you for your enquiry. This antibody has been characterised with others raised against NMYC in the following paper which may be of help to you:

Identification and characterisation of the NMYC gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. Ikegaki N et al. Prodedings of National Acadamy of Science USA. 83 p5929.

This indiates that epitope for this antibody lies in the second exon of NMYC and it will recognise the cleavage products from NMYC.

I hope this helps. Please get in touch again if you require any more advice.

With thanks

The recommended dilutions of ab16898 for IHC-Fr is at a concentration of 1 - 5 ug/ml,but in my research the antibody is unstability(5-10ug/ml).The first preliminary experiment was very good. The second and the third have failed.So what should I do?Could you give me some suggestions?Thank you for your help!

ANSWER:

Thank you for contacting us for technical support. The problem you are experiencing may be due to the fact that the antibody is very fragile. We recommend to aliquot and store at -20°C upon arrival. Can you tell me how you proceeded? If you can also tell me your order details I will look at the shipping details to investigate if the antibody suffered from a delay.

I look forward to hearing from you to resolve this matter,

Thank you for your time yesterday discussing the antibody problem .

The researcher informed us that the Abcam antibody 16898 was diluted as recommended with PBS, and was stored at 4 degrees. After about two weeks a yellow deposit has appeared and the fluid is cloudy. According to the user, the instructions that came with the antibody clearly said not to add preservatives.

This is the first antibody they use that got contaminated.

ANSWER:

Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below:

Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate.

Furthermore we recommend that the antibody is stored at -20°C and that freeze / thaw cycles are avoided. Again as stated below:

Storage instructions: Aliquot and store at -20°C. Avoid freeze / thaw cycles.

We DO recommend that the antibody is diluted in PBS for certain applications prior to use. However, this certainly would NOT be recommended if the intention is to store the antibody in the fridge for two weeks, especially given the lack of preservative. It is therefore of no surprise to me that the antibody has developed mould ("contaminated").

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Is it possible to test the n-Myc (ab16898) antibody for you? Can you send us a small amount to test?

ANSWER:

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).