n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)

Product Name 

n-Myc antibody [NCM II 100] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [NCM II 100] to n-Myc - ChIP Grade

Immunogen 

Recombinant fusion protein (Human).

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody reacts with N-myc encoded proteins and their cleavage products.

Tested applications 
(see key)

ChIP, ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution. 2 µg PMID: 19495417
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-Fr: Use at a concentration of 1 - 5 µg/ml.
IP: Use at a concentration of 1 - 2 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IMR5 Cells

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

Relevance 

The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of 64 kDa and 67 kDa that exhibit relatively short (30 minute) half lives in vivo and exhibit DNA binding properties in vitro. Members of the Myc gene family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

NCM II 100

Myeloma 

Sp2/0

Isotype 

IgG1

Light chain type 

kappa

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 0.05M Sodium phosphate

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.

See below for n-Myc antibody [NCM II 100] - ChIP Grade images, references, products related to ab16898 and other tools.

n-Myc antibody [NCM II 100] - ChIP Grade images:

References for n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)

  This product has been used in: (two most recent references)

Cotterman R & Knoepfler PS N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b. PLoS One 4:e5799 (2009). ChIP; Human. PubMed: 19495417

Martins RA et al. N-myc coordinates retinal growth with eye size during mouse development. Genes Dev 22:179-93 (2008). WB; Mouse. PubMed: 18198336

See all 4 publication references for this product.


If you publish research using ab16898 please let us know so that we can cite the reference on this datasheet.

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