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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Overview
Product name
Anti-n-Myc antibody
See all n-Myc products (4) ...
Description
Rabbit polyclonal to n-Myc
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc.
(Peptide available as ab31595.)
Positive control
This antibody gave a positive signal in the following lysates: Heart (Human) Whole Cell Lysate - fetal normal tissue Heart (Mouse) Tissue Lysate Skeletal Muscle (Mouse) Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF: Use at an assay dependent concentration. (PubMed: 22216302)
Target
Function
May function as a transcription factor.
Involvement in disease
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Developmental stage
Expressed during fetal development.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP04198
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
Database links
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Entrez Gene: 298894 Rat
- Omim: 164840 Human
- SwissProt: P18444 Chicken
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- SwissProt: Q63379 Rat
see all
Anti-n-Myc antibody images:
Western blot - n-Myc antibody (ab24193)
All lanes : Anti-n-Myc antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 25 kDa (possible cleavage fragment,cross reactivity),33 kDa (possible cleavage fragment,cross reactivity),39 kDa (possible cleavage fragment,cross reactivity).
Western blot - n-Myc antibody (ab24193)
All lanes : Anti-n-Myc antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-n-Myc antibody (ab24193)
This product has been referenced in:
- Anedda Fet al. Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1). PLoS One 6:e29523 (2011). ICC/IF; Human.Read more (PubMed: 22216302) »
See 1 publication for this product
Publishing research using ab24193? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"