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Western blot - n-Myc antibody (ab24193)
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Western blot - n-Myc antibody (ab24193)
(enlarge)
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Product Name
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n-Myc antibody
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See all n-Myc antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to n-Myc
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc.
(Peptide available as ab31595.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Chk, NHuPrm
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
Heart (Human) Whole Cell Lysate - fetal normal tissue
Heart (Mouse) Tissue Lysate
Skeletal Muscle (Mouse) Tissue Lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Relevance
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The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of 64 kDa and 67 kDa that exhibit relatively short (30 minute) half lives in vivo and exhibit DNA binding properties in vitro. Members of the Myc gene family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this n-Myc antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for n-Myc antibody images, references, products related to ab24193 and other tools.
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n-Myc antibody images:
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 Western blot - n-Myc antibody (ab24193)
All lanes : n-Myc antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 25 kDa (possible cleavage fragment,cross reactivity),33 kDa (possible cleavage fragment,cross reactivity),39 kDa (possible cleavage fragment,cross reactivity).
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Search PubMed (MEDLINE) for references to n-Myc
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n-Myc antibody - more information
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Customer reviews (feedback) regarding n-Myc antibody |
Customer FAQs regarding n-Myc antibody |
Protocols for n-Myc antibody |
Price and availability of products related to n-Myc antibody |
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n-Myc antibody - related products:
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n-Myc antibody - other tools:
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Contact Abcam with a Technical Enquiry about n-Myc antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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