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Overview

  • Product nameAnti-n-Myc antibodySee all n-Myc primary antibodies ...
  • Description
    Rabbit polyclonal to n-Myc
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab31595.)

  • Positive controlThis antibody gave a positive signal in the following lysates: Heart (Human) Whole Cell Lysate - fetal normal tissue Heart (Mouse) Tissue Lysate Skeletal Muscle (Mouse) Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab24193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with n-Myc peptide (ab31595).
ICC/IF ICC/IF: Use at an assay dependent concentration. PubMed: 22216302

Target

  • FunctionMay function as a transcription factor.
  • Involvement in diseaseNote=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stageExpressed during fetal development.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P04198 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      bHLHe37 antibodyClass E basic helix-loop-helix protein 37 antibodyMODED antibody
      MYCN antibodyMYCN_HUMAN antibodyN myc antibodyN myc proto oncogene protein antibodyN-myc proto-oncogene protein antibodyNeuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibodyNeuroblastoma MYC oncogene antibodyNMYC antibodyNMYC proto oncogene protein antibodyODED antibodyOncogene NMYC antibodypp65/67 antibodyv myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibodyv myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Anti-n-Myc antibody images

  • Western blot - n-Myc antibody (ab24193)
    Western blot - n-Myc antibody (ab24193)
    All lanes : Anti-n-Myc antibody (ab24193) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
    Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 25 kDa (possible cleavage fragment,cross reactivity),33 kDa (possible cleavage fragment,cross reactivity),39 kDa (possible cleavage fragment,cross reactivity).
  • Western blot - n-Myc antibody (ab24193)
    Western blot - n-Myc antibody (ab24193)
    All lanes : Anti-n-Myc antibody (ab24193) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-n-Myc antibody (ab24193)

This product has been referenced in:
  • Anedda F  et al. Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1). PLoS One 6:e29523 (2011). ICC/IF ; Human . Read more (PubMed: 22216302) »

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Western blot abreview for Anti-n-Myc antibody

 Good
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 30 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Dec 30 2010

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