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Anti-n-Myc antibody (ab24339)

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2 questions for ab24339

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Question 1

Monday 03-April-2006

Enclose please find the woodchuck N-Myc protein sequence. Please help me to do the alignment to make sure the antibody can work. Thanks for your help. I am looking forward to hearing from you soon.

MRSCTVSTMPRMICRNADLEFDWLQPCFYPDEDDFYFSGPNSTPPGEDIWKKFELLPTPPLSPSCAFLELSTEPSDWASE MMLTEADLWGNPDEEDVFGPGGLGSLTPNPVILRDCMWSGFSAREKLERAMSEKMQHGHEPAATGPATQVPGAGAASPAG RGHSGTAGAALPAELAHPAAECVDPAVVFLLPVSKRNPVPVRVAPARAPARASAVGAAVARAAAPATAAVAAPPGLSSRP PNGGDHKVLSTSGEDALSDEVDEEEDEEEEIDVVTVEKSCKTGGTTFTLTVSPKNTALGLGREQSRELILQRSVPIYQQH NYAAPSPYVESEDAPPQKKIKREVSPHPLKSVIHPKGKSFSPRKSDSEDSVRRRNHNILERQRRNDLRSSFTTLRDHVPE LVKNEKAAKVVILKKACEYVHYLQAKEHQLLMEKEKLQARQQQLLKIIELAWTF.

ANSWER:

 

Thank you for your enquiry.

Following an alignment of the immunogen sequence with the woodchuck sequence that you have provided me with I can tell you that there was no sequence similarity and therefore I predict that this antibody would not react against the woodchuck homolog.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 2

Friday 31-March-2006

Would you kindly inform me the Synthetic peptide sequence of n-Myc antibody (ab24339)? I am looking for a anti-N-Myc polyclonal antibody to stain woodchuck liver slides. I am looking forward to hearing from you soon.

ANSWER:

 

Thank you for your enquiry.

Unfortunately the immunogen sequence used to raise n-Myc antibody (ab24339) remains proprietary. It was a synthetic peptide corresponding to a region of Human MYCN. However, if you provide me with the sequence or Swiss-Prot ID I can request that our lab performs an alignment of the immunogen against your sequence of the woodchuck homolog.

I look forward to hearing from you.

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