Anti-N acetylglucosamine kinase antibody (ab95182)

Overview

  • Product nameAnti-N acetylglucosamine kinase antibody
    See all N acetylglucosamine kinase primary antibodies
  • Description
    Rabbit polyclonal to N acetylglucosamine kinase
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 50 and 100 of Human N acetylglucosamine kinase (NP_060037.2)

  • Positive control
    • Whole cell lysate from HeLa cells, 293T cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionConverts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the eukaryotic-type N-acetylglucosamine kinase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.1.59 antibody
    • GlcNAc kinase antibody
    • GNK antibody
    • HSA242910 antibody
    • N acetylglucosamine kinase antibody
    • N-acetyl-D-glucosamine kinase antibody
    • N-acetylglucosamine kinase antibody
    • nagK antibody
    • NAGK_HUMAN antibody
    see all

Anti-N acetylglucosamine kinase antibody images

  • All lanes : Anti-N acetylglucosamine kinase antibody (ab95182) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa at 50 µg
    Lane 2 : Whole cell lysate from HeLa at 15 µg
    Lane 3 : Whole cell lysate from HeLa at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 37 kDa


    Exposure time : 30 seconds
  • Detection of Human N acetylglucosamine kinase by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) N acetylglucosamine kinase was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 30 seconds.

References for Anti-N acetylglucosamine kinase antibody (ab95182)

ab95182 has not yet been referenced specifically in any publications.

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