Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
Western blot - N acetylglucosamine kinase antibody (ab95182)
All lanes : Anti-N acetylglucosamine kinase antibody (ab95182) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa at 50 µg Lane 2 : Whole cell lysate from HeLa at 15 µg Lane 3 : Whole cell lysate from HeLa at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 37 kDa
Exposure time : 30 seconds
Immunoprecipitation - N acetylglucosamine kinase antibody (ab95182)
Detection of Human N acetylglucosamine kinase by Immunoprecipitation and Western Blot.
Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded.
ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2)
N acetylglucosamine kinase was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3).
Detection: Chemiluminescence with exposure times of 30 seconds.