Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: 1/7000 - 1/35000. This product has been assayed against 1µg of N-Acylmannoseamine-1Dehydrogenase in a standard capture ELISA using Peroxidase conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceN acylmannosamine 1 dehydrogenase belongs to the short chain dehydrogenases/ reductases family (SDR). It acts on acetyl D mannosamine and glycolyl D mannosamine.
NAM DH antibody
UDP-N-acetyl D mannosamine dehydrogenase antibody
References for Anti-N-acylmannosamine 1-dehydrogenase antibody (Biotin) (ab34542)
has not yet been referenced specifically in any publications.
Publishing research using ab34542? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"