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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-NAB1 antibody
See all NAB1 products (3) ...
Description
Rabbit polyclonal to NAB1
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
A region within synthetic peptide: ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA, corresponding to amino acids 71-120 of Human NAB1
ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA
Positive control
Human intestine, transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Titre using peptide based assay 1/62500.
IHC-P: Use at a concentration of 16 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
Tissue specificity
Isoform Short is found in a myeloid leukemia cell line.
Sequence similarities
Belongs to the NAB family.
Domain
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EGR 1 binding protein 1 antibody
- EGR-1-binding protein 1 antibody
- NAB1 antibody
see all
Database links
- Entrez Gene: 4664 Human
- Entrez Gene: 17936 Mouse
- Entrez Gene: 64824 Rat
- Omim: 600800 Human
- SwissProt: Q13506 Human
- SwissProt: Q61122 Mouse
- SwissProt: Q62722 Rat
- Unigene: 723892 Human
see all
Anti-NAB1 antibody images:
Western blot - NAB1 antibody (ab50381)
Anti-NAB1 antibody (ab50381) at 1 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
Immunohistochemistry (Paraffin-embedded sections) - NAB1 antibody (ab50381)
Ab50381 at 16µg/ml staining epithelial cells of human intestinal villus; paraffin embedded; magnification x400
References for Anti-NAB1 antibody (ab50381)
ab50381 has not yet been referenced specifically in any publications.
Publishing research using ab50381? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"