Endogenous inhibitor of GLYT2 with little or no effect on GLYT1 and is analgesic in rat models of neuropathic and inflammatory pain. Anandamide-like compound, with little affinity at CB1 receptors, VR1 receptors and anandamide transporters. Potent inhibitor of T-type Ca2+ channels with little activity on anandamide-sensitive TRPV1 and TASK1 currents and high-voltage-activated (HVA) Cav1.2, Cav2.2, Nav1.7, and Nav1.8 currents.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab32557 staining p38 (phospho T180 + Y182) in RAW 264.7 cells treated with N-Arachidonylglycine (ab120346), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of N-Arachidonylglycine, as described in literature. The cells were incubated at 37°C for 1h in media containing different concentrations of ab120346 (N-Arachidonylglycine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for N-Arachidonylglycine (NAGly) (ab120346)