Overview

  • Product nameAnti-N Cadherin antibodySee all N Cadherin primary antibodies ...
  • Description
    Rabbit polyclonal to N Cadherin
  • Specificityab76057 does not crossreact with E Cadherin.
  • Tested applicationsIHC-P, WB, IP, ELISA, ICC/IF, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human
    Predicted to work with: Rat, Chicken, Chimpanzee
  • Immunogen

    Unphosphorylated synthetic peptide: ERPIHAEPQYPVRS, corresponding to amino acids 811-824 of Human N Cadherin (coupled to a carrier protein). This sequence is conserved in rat and mouse N cadherin, and has three amino acid differences from the conserved region in R Cadherin.

  • Positive control
    • WB: Human endothelial cells (untreated or treated with pervanadate (1 mM) for 30 minutes) and mouse brain tissue. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab76057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 100 kDa.
IP 1/100.
ELISA 1/2000.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P19022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural Cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin antibody images

  • ab76057 staining N Cadherin in human BicR19 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100) for 30 minutes. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
    Gating Strategy: Oral epithelial cells.

    See Abreview

  • All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilution

    Lane 1 : Human endothelial cells untreated
    Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min


    Predicted band size : 100 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab76057 at 1:100, staining differentiated embryonic stem cells by ICC/IF

    See Abreview

  • All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilution

    Lane 1 : MDA-MB-231 cells (Negative Control)
    Lane 2 : U2OS cells (Positive Control)

    Lysates/proteins at 200000 cells per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of N Cadherin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-N Cadherin antibody (ab76057)

This product has been referenced in:
  • Jordan NV  et al. SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls epithelial-mesenchymal transition by inducing Wnt5a signaling. Mol Cell Biol 33:3011-25 (2013). Read more (PubMed: 23716599) »
  • Chang CJ  et al. p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs. Nat Cell Biol 13:317-23 (2011). WB ; Human . Read more (PubMed: 21336307) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Human Cell (BicR19)
Specification BicR19
Gating Strategy oral epithelial cells
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: PBS
Username

Ms. Zahra Abdalla

Verified customer

Submitted Mar 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 1% serum + 0.1% BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step None
Sample Human Tissue sections (oral squamous cell carcinoma)
Specification oral squamous cell carcinoma
Permeabilization No
Fixative Paraformaldehyde
Username

Ms. Zahra Abdalla

Verified customer

Submitted Feb 26 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Rat vascular smooth muscle cells)
Specification Rat vascular smooth muscle cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Sheep Tissue lysate - whole (Cardiac)
Specification Cardiac
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 19 2013

Application Flow Cytometry
Sample Human Cell (A549)
Specification A549
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Live cells
Username

Abcam user community

Verified customer

Submitted Jan 21 2013

Application Western blot
Sample Human Cell lysate - whole cell (A549 Lung adenocarcinoma cell line)
Loading amount 20 µg
Specification A549 Lung adenocarcinoma cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Ms. Zahra Abdalla

Verified customer

Submitted Dec 20 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (oral squamous cell carcinoma)
Specification oral squamous cell carcinoma
Fixative Paraformaldehyde
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Ms. Zahra Abdalla

Verified customer

Submitted Dec 12 2012

Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 100000 cells
Specification A549
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (schwann cells)
Loading amount 25 µg
Specification schwann cells
Gel Running Conditions Reduced Denaturing (8% SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Schwann cell)
Specification Schwann cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% TX-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Xinpeng Dun

Verified customer

Submitted Jul 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"