Recombinant
RabMAb

Anti-N Cadherin antibody [EPR1791-4] (ab76011)

Overview

  • Product name
    Anti-N Cadherin antibody [EPR1791-4]
    See all N Cadherin primary antibodies
  • Description
    Rabbit monoclonal [EPR1791-4] to N Cadherin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human N Cadherin aa 150-250 (extracellular).

  • Positive control
    • Huvec, C6 and HeLa cell lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 100 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
    • Sequence similarities
      Contains 5 cadherin domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CADH2_HUMAN antibody
      • Cadherin 2 antibody
      • Cadherin 2 N cadherin neuronal antibody
      • Cadherin 2 type 1 antibody
      • Cadherin 2 type 1 N cadherin neuronal antibody
      • Cadherin 2, type 1, N-cadherin (neuronal) antibody
      • Cadherin-2 antibody
      • Cadherin2 antibody
      • Calcium dependent adhesion protein neuronal antibody
      • CD325 antibody
      • CD325 antigen antibody
      • CDH2 antibody
      • CDHN antibody
      • CDw325 antibody
      • CDw325 antigen antibody
      • N cadherin 1 antibody
      • N-cadherin antibody
      • NCAD antibody
      • Neural cadherin antibody
      • OTTHUMP00000066304 antibody
      • OTTHUMP00000067378 antibody
      see all

    Anti-N Cadherin antibody [EPR1791-4] images

    • ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Lanes 1 - 2 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/20000 dilution
      Lane 3 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/5000 dilution

      Lane 1 : Huvec cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    References for Anti-N Cadherin antibody [EPR1791-4] (ab76011)

    This product has been referenced in:
    • Xiong Y  et al. Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma. Oncotarget 8:24902-24914 (2017). Human . Read more (PubMed: 28212546) »
    • Hwang PY  et al. N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. Sci Rep 6:28038 (2016). IHC-P . Read more (PubMed: 27292569) »

    See all 10 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (oviductal tissues)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
    Permeabilization
    No
    Specification
    oviductal tissues
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Yoshihiko Kobayashi

    Verified customer

    Submitted Sep 21 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF-7 cell)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    MCF-7 cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 11 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic mouse brain coronal cortical section)
    Permeabilization
    Yes - Triton X 100
    Specification
    Embryonic mouse brain coronal cortical section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Bhavin Shah

    Verified customer

    Submitted Oct 20 2015

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Specification
    MEFs
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 16 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    1%BSA 1%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Sample
    Mouse Tissue sections (Pancreas)
    Specification
    Pancreas
    Permeabilization
    Yes - TBS-Tween 0,1%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEFs wild type)
    Specification
    MEFs wild type
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,3% Triton PBS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2013

    Thank you for contacting us and your interest in our products.
    The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

    Read More

    Thank you for your reply.

    Can you please confirm what species your samples are from, including cell line or tissue type?

    Also what is the order number for this product? I will need to ensure that the order is not more than 6 months ...

    Read More

    Thank you for your reply.

    You're having trouble with the ab76011, anti-N cadherin antibody, correct? Not the ab1791 histone H3? You had included ab76011 in your initial email, but ab1791 in your reply.

    Do you have a lot # from the via...

    Read More

    Thank you very much for your interest in Abcam antibodies.

    We have 2 rabbitantibodies directed against the extracellular part of the N-cadherin :

    http://www.abcam.com/ab76011, rabbit monoclonal, tested in Western Blot, ICC/IF, Flow ...

    Read More

    1-10 of 17 Abreviews or Q&A

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