Anti-N Cadherin (phospho Y860) antibody (ab119752)

Overview

  • Product nameAnti-N Cadherin (phospho Y860) antibody
    See all N Cadherin primary antibodies
  • Description
    Rabbit polyclonal to N Cadherin (phospho Y860)
  • SpecificityImmunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733).
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phospho-peptide coupled to acarrier protein, corresponding to amino acids surrounding tyrosine 860 in Human N Cadherin.

  • Positive control
    • C2C12 and Human endothelial cells treated with pervanadate.
  • General notesDo not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa).
ICC 1/200.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin (phospho Y860) antibody images

  • ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
    Detection used an appropriate secondary antibody conjugated to Cy3.
  • Lanes 1 - 2 : Anti-N Cadherin (Cytoplasmic) at 1/1000 dilution
    Lanes 3 - 4 : Anti-N Cadherin (phospho Y820) at 1/1000 dilution
    Lanes 5 - 6 : Anti-N Cadherin (a.a. 853-864) at 1/1000 dilution
    Lanes 7 - 8 : Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution

    Lane 1 : Human endothelial cells, untreated
    Lane 2 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min
    Lane 3 : Human endothelial cells, untreated
    Lane 4 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min
    Lane 5 : Human endothelial cells, untreated
    Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
    Lane 7 : Human endothelial cells, untreated
    Lane 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min


    Predicted band size : 99 kDa

References for Anti-N Cadherin (phospho Y860) antibody (ab119752)

ab119752 has not yet been referenced specifically in any publications.

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