Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)


  • Product nameAnti-N epsilon gamma glutamyl Lysine antibody [81D4]
  • Description
    Mouse monoclonal [81D4] to N epsilon gamma glutamyl Lysine
  • SpecificityReacts with Ne (g-L-glutamyl)-L-lysine. Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. There is no cross reaction with: - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties
  • Tested applicationsSuitable for: IHC-P, ELISA, IHC-Frmore details
  • Immunogen

    Synthetic isopeptide.

  • General notesThe ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.



Our Abpromise guarantee covers the use of ab424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/800.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/800.

Anti-N epsilon gamma glutamyl Lysine antibody [81D4] images

  • ab424 staining N epsilon gamma glutamyl Lysine in skin tissue sections.
  • ab424 staining N epsilon gamma glutamyl Lysine in kidney tissue sections by Immunohistochemistry (Frozen sections). Sections A through D were stained for insoluble tissue transglutaminase, while E through H were stained for N epsilon gamma glutamyl Lysine crosslink. A, B, E, F were control kidneys; C, D, G, H were diseased kidneys showing increased staining in the expanded interstitium, the mesangium and periglomerular.

References for Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)

This product has been referenced in:
  • Witsch TJ  et al. Transglutaminase 2: a new player in bronchopulmonary dysplasia? Eur Respir J 44:109-21 (2014). Read more (PubMed: 24603819) »
  • Assi J  et al. Transglutaminase 2 overexpression in tumor stroma identifies invasive ductal carcinomas of breast at high risk of recurrence. PLoS One 8:e74437 (2013). IHC-P . Read more (PubMed: 24058567) »

See all 10 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Trabecular Meshwork)
Permeabilization No
Specification Trabecular Meshwork
Blocking step PBS superblock blocking buffer (Thermo Scientific, Prod # 37517) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 21 2015

Thank you very much for keeping me updated.

I'm glad to hear that the results seem to have improved. We currently have only that lot in stock (2 vials in our US stock and 1 vial in our UK stock), so there should be consistency in results. Read More

Thank you very much for your reply.

I'm sending a free of charge vial of ab97230 on the order ***, which should ship out tonight and arrive tomorrow.

I've also included the promo code for the cell imaging book.

Please keep...

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Thank you very much for your reply.

For your assay, either of these antibodies will be suitable. I would suggest ab97230 because it is in stock and we can ship it to you overnight. Ab98679 is back-ordered and I expect it to work similarly to ...

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Thank you very much for your reply.

I apologize but we won't be able to test the antibody with your samples in our lab at this time. I'm very sorry for the inconvenience. Did you check whether the mouse-on-mouse HRP polymer detection kit work...

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Thank you very much for keeping me updated about the results.

I'm sorry to hear that altering the protocol did not help improve the results. Have you also tried staining sections without doing antigen retrieval at all? I apologize that we don...

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Thank youvery much for your reply and for sending these additional details.

I have a couple of suggestions that might help improve the results. It may be useful to decrease the antigen retrieval time to 15-20 minutes, in order to reduce the r...

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Thank you very much for your reply, and for your patience.

Thank you also for sending the images of your staining results. I've reviewed some of the literature in which this antibody was used in IHC-P, and there aren't many more details about...

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Thank you very much for your call last week and for your patience while I've looked into your enquiry.

We have not tested this antibody in our own labs in IHC on formalin-fixed paraffin-embedded tissue, but it has been testedin IHC-P accordin...

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I can confirm that this antibody will detect N epsilon gamma glutamyl Lysine also if part of a human protein. In the publication PubMed ID 18235007 for example, researchers have used this antibody successfully in human paraffin embedded tissue slides. ...

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