The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 50 kDa.
1/1000. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMay function as a transcription factor.
Involvement in diseaseNote=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses. Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described. Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
Overlay histogram showing SH-SH5Y cells stained with ab119701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119701, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-n-Myc antibody [6A4I] (ab119701)
All lanes : Anti-n-Myc antibody [6A4I] (ab119701) at 1/2000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Ramos cell lysate Lane 3 : HuT78 cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Mouse Brain tissue lysate Lane 6 : Mouse Testis tissue lysate Lane 7 : Rat Brain tissue lysate Lane 8 : Rat Testis tissue lysate
Predicted band size : 50 kDa
References for Anti-n-Myc antibody [6A4I] (ab119701)
has not yet been referenced specifically in any publications.
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