Anti-n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)

  Immunoprecipitation - n-Myc antibody [NCM II 100] (ab16898)

Immunoprecipitation of N-Myc from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
ab16898 used at 1 mg/sample.

  Flow Cytometry - n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)

Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.