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Fusion protein corresponding to Human n-Myc. Recombinant fusion protein.
Original clone reference: PubMed ID - 2426708.
Database link: P04198
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product within the next few months as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab24193 or ab189528 as replacements.
Our Abpromise guarantee covers the use of ab16898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 - 5 µg/ml.
|IP||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).
|ICC/IF||Use a concentration of 1 - 5 µg/ml.
Pubmed ID: 2426708
|Flow Cyt||Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ChIP||Use at an assay dependent concentration. PubMed: 19495417Suggested to be used at 2µg.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"