Overview

  • Product nameAnti-N myc interactor antibody
    See all N myc interactor primary antibodies
  • Description
    Rabbit polyclonal to N myc interactor
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 (MEADKDDTQQ ILKEHSPDE F IKDEQNKG LI DEITKKN IQL KKEIQK LETE) of human N myc interactor

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceNMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • N myc (and STAT) interactor antibody
      • N myc and STAT interactor antibody
      • N myc interactor antibody
      • NMI antibody
      • NMYC and STAT interactor antibody
      • NMYC interactor antibody
      see all

    Anti-N myc interactor antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling N myc interactor with ab70209 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Immunoprecipitation of Human N myc interactor. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NMI antibody (ab70209) used at 3 µg/ml for IP. Arrows indicate N myc interactor on Lane 1. lane 2 represents IgG IP control. Note: ab70210 at 0.04 µg/ml was used for WB detection.

    References for Anti-N myc interactor antibody (ab70209)

    ab70209 has not yet been referenced specifically in any publications.

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