The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
RelevanceNMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling N myc interactor with ab70209 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - N myc interactor antibody (ab70209)
Immunoprecipitation of Human N myc interactor. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NMI antibody (ab70209) used at 3 µg/ml for IP. Arrows indicate N myc interactor on Lane 1. lane 2 represents IgG IP control.
Note: ab70210 at 0.04 µg/ml was used for WB detection.
References for Anti-N myc interactor antibody (ab70209)
has not yet been referenced specifically in any publications.
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