The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
Application notesIs unsuitable for IP.
RelevanceNMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and bladder carcinoma (right) tissues labelling N myc interactor with ab70210 at 1/1000 (0.2 µg/ml). Detection: DAB.
Western blot - N myc interactor antibody (ab70210)
All lanes : Anti-N myc interactor antibody (ab70210) at 0.04 µg/ml
Lane 1 : Whole
cell lysate from HeLa cells at 5 µg Lane 2 : Whole
cell lysate from HeLa cells at 15 µg Lane 3 : Whole
cell lysate from HeLa cells at 50 Lane 4 : Whole
cell lysate from 293T cells at 50 Lane 5 : Whole
cell lysate from NIH3T3 cells at 50