• Product nameAnti-N myc interactor antibody
    See all N myc interactor primary antibodies
  • Description
    Rabbit polyclonal to N myc interactor
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 250 and 307 of human N myc interactor. (NP_004679.1)

  • Positive control
    • Whole cell lysate from HeLa cells.



Our Abpromise guarantee covers the use of ab70210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • RelevanceNMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • N myc (and STAT) interactor antibody
      • N myc and STAT interactor antibody
      • N myc interactor antibody
      • NMI antibody
      • NMYC and STAT interactor antibody
      • NMYC interactor antibody
      see all

    Anti-N myc interactor antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and bladder carcinoma (right) tissues labelling N myc interactor with ab70210 at 1/1000 (0.2 µg/ml). Detection: DAB.
    • All lanes : Anti-N myc interactor antibody (ab70210) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50
      Lane 4 : Whole cell lysate from 293T cells at 50
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50

      Predicted band size : 35 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

      Exposure time : 30 seconds

    References for Anti-N myc interactor antibody (ab70210)

    ab70210 has not yet been referenced specifically in any publications.

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