Validated using a knockout cell line
Recombinant
RabMAb

Anti-N myc interactor antibody [EPR11065(2)] (ab183724)

Overview

  • Product name
    Anti-N myc interactor antibody [EPR11065(2)]
    See all N myc interactor primary antibodies
  • Description
    Rabbit monoclonal [EPR11065(2)] to N myc interactor
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human N myc interactor aa 1-100. The exact sequence is proprietary.
    Database link: Q13287

  • Positive control
    • HeLa cells and cell lysates; K562 and HepG2 cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50.
ICC/IF 1/100.
IP 1/40 - 1/60.

Target

  • Relevance
    NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • N myc (and STAT) interactor antibody
    • N myc and STAT interactor antibody
    • N myc interactor antibody
    • NMI antibody
    • NMYC and STAT interactor antibody
    • NMYC interactor antibody
    see all

Images



  • Predicted band size : 35 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2:
    N myc interactor knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4:
    Merged signal (red and green). Green - ab183724 observed at 39 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab183724 was shown to specifically react with N myc interactor when N myc interactor knockout samples were used. Wild-type and N myc interactor knockout samples were subjected to SDS-PAGE. ab183724 and ab18058 (loading control to Vinculin) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-N myc interactor antibody [EPR11065(2)] (ab183724) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 35 kDa
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling N myc interactor with ab183724 at 1/50 dilution. The slide is counterstained with Hematoxylin.

  • Immunofluorescence analysis of acetone-fixed HeLa cells labeling N myc interactor with ab183724 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody (green). The slide on the right is stained with Dapi (blue).

  • Western blot analysis of K562 cell lysate precipitated with ab183724 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution was then used. The blocking buffer and dilution buffer was 5% NFDM/TBST.

References

ab183724 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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