Overview

  • Product nameAnti-N ras + c Ha ras antibody
  • Description
    Sheep polyclonal to N ras + c Ha ras
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    MTEYKLVVVGAGGVGKSA

    conjugated to KLH, corresponding to N terminal amino acids 1-18 of Human N ras.

  • General notes


    The antibody detects a major protein band at 21 kDa from detergent lysates produced from N866 (N-ras overproducing), EC816 (c-Ha-ras over-producing) cell lines and HL-60 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 21 kDa.

Target

  • RelevanceRAS - the RAS superfamily comprises around 50 related genes encoding GTP-binding domain (G-proteins) involved in signal transduction. The main genes are HRAS, NRAS and KRAS. Ras proteins are membrane-bound GTPases. The inactive form is GDP-bound. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, colony-stimulating factor and fibroblast growth factor. These kinases transiently convert RAS-GDP to RAS-GTP, the active form of RAS. Single amino acid substitutions can activate RAS making it highly oncogenic. Such mutations generally reduce the GTPase activity of RAS, prolonging it in its active GTP-bound form. The consequence of this is sustained activation of the RAF1-MAPK signalling pathway. RAS mutations are found in 10-15% of tumours. A high incidence of RAS mutations is found in pancreatic cancers.
  • Cellular localizationPlasma membrane associated.
  • Database links
  • Alternative names
    • ALPS4 antibody
    • GTPase HRas antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v-ras) oncogene homolog antibody
    • NRAS antibody
    • Ras family small GTP binding protein H Ras antibody
    • RAS1 antibody
    • Transforming protein p21 antibody
    see all

References for Anti-N ras + c Ha ras antibody (ab24043)

ab24043 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"