Anti-N WASP (phospho S484 + S485) antibody (ab77852)

Overview

  • Product name
    Anti-N WASP (phospho S484 + S485) antibody
    See all N WASP primary antibodies
  • Description
    Rabbit polyclonal to N WASP (phospho S484 + S485)
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH, corresponding to amino acid residues around serine 484 and 485 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.

  • Positive control
    • Jurkat cell lysate, rat brain, A431, human endothelial and SKN-SH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 63, 65 kDa (predicted molecular weight: 55 kDa).

The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

ELISA 1/2000.

Target

  • Function
    Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
  • Sequence similarities
    Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 2 WH2 domains.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779G0847 antibody
    • MGC48327 antibody
    • N-WASP antibody
    • Neural Wiskott Aldrich syndrome protein antibody
    • Neural Wiskott-Aldrich syndrome protein antibody
    • NWASP antibody
    • Wasl antibody
    • WASL_HUMAN antibody
    • WASPB antibody
    • Wiskott Aldrich syndrome gene like antibody
    • Wiskott Aldrich syndrome gene like protein antibody
    • Wiskott Aldrich syndrome like antibody
    • WiskottAldrich syndrome like antibody
    see all

Images

  • All lanes : Anti-N WASP (phospho S484 + S485) antibody (ab77852) at 1/1000 dilution

    Lane 1 : Neonatal rat brain lysate
    Lane 2 : Neonatal rat brain lysate treated with alkaline phosphatase.

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References

ab77852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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