Synthetic peptide (Human) conjugated to KLH, corresponding to amino acid residues around serine 484 and 485 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
Jurkat cell lysate, rat brain, A431, human endothelial and SKN-SH cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 63, 65 kDa (predicted molecular weight: 55 kDa).
The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.