Anti-N WASP (phospho Y256) antibody (ab23395)
Key features and details
- Rabbit polyclonal to N WASP (phospho Y256)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-N WASP (phospho Y256) antibody
See all N WASP primary antibodies -
Description
Rabbit polyclonal to N WASP (phospho Y256) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human N WASP (phospho Y256).
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Positive control
- Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.
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Notes |
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WB
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine. |
Target
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Function
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. -
Sequence similarities
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 8976 Human
- Omim: 605056 Human
- SwissProt: O00401 Human
- Unigene: 143728 Human
- Unigene: 83916 Human
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Alternative names
- DKFZp779G0847 antibody
- MGC48327 antibody
- N-WASP antibody
see all
Images
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All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution
Lane 1 : Control A431 cells 20ug/lane
Lane 2 : Pervanadate-treated A431 cells 20 ug/lane
Predicted band size: 55 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab23395 has been referenced in 6 publications.
- Du G et al. Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo. Cell Death Dis 11:118 (2020). PubMed: 32051399
- Zhang W et al. Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle. J Physiol 596:3617-3635 (2018). PubMed: 29746010
- Zhang W et al. p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization. J Physiol 594:4879-900 (2016). PubMed: 27038336
- Rajput C et al. Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. J Biol Chem 288:4241-50 (2013). WB ; Human . PubMed: 23212915
- Chacón MR et al. Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth. Development 139:3200-10 (2012). WB . PubMed: 22872087
- Zhang W et al. The small GTPase RhoA regulates the contraction of smooth muscle tissues by catalyzing the assembly of cytoskeletal signaling complexes at membrane adhesion sites. J Biol Chem 287:33996-4008 (2012). WB ; Dog . PubMed: 22893699