Overview

  • Product nameAnti-N4BP1 antibody
    See all N4BP1 primary antibodies
  • Description
    Rabbit polyclonal to N4BP1
  • SpecificityAt least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform.
  • Tested applicationsICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).

  • Positive control
    • HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at 4°C, stable for one year. Should not be exposed to prolonged high temperatures. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab106649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.

Target

  • FunctionInhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • Tissue specificityDetected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • Sequence similaritiesBelongs to the N4BP1 family.
  • Post-translational
    modifications
    Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody images

  • Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.
  • All lanes : Anti-N4BP1 antibody (ab106649) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 100 kDa
  • Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.

References for Anti-N4BP1 antibody (ab106649)

ab106649 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106649.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"