• Product name
  • Description
    Rabbit polyclonal to N4BP1
  • Specificity
    This antibody detects endogenous levels of total N4BP1 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal region of human N4BP1.

  • Positive control
    • Extracts from MCF-7 cells, 293 cells and Jurkat cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab74260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24831002
WB 1/500 - 1/1000. Predicted molecular weight: 100 kDa.
ELISA 1/40000.


  • Function
    Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • Tissue specificity
    Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • Sequence similarities
    Belongs to the N4BP1 family.
  • Post-translational
    Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody images

  • All lanes : Anti-N4BP1 antibody (ab74260) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from Jurkat cells
    Lane 4 : Extracts from MCF-7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa

References for Anti-N4BP1 antibody (ab74260)

This product has been referenced in:
  • Sugeno N  et al. Lys-63-linked Ubiquitination by E3 Ubiquitin Ligase Nedd4-1 Facilitates Endosomal Sequestration of Internalized a-Synuclein. J Biol Chem 289:18137-18151 (2014). WB, ICC/IF . Read more (PubMed: 24831002) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed aga...

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Thank you for contacting Abcam regarding ab74260. I am sorry that you are experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with...

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