Anti-N6-methyladenosine (m6a) antibody (ab151230)

Overview

  • Product name
    Anti-N6-methyladenosine (m6a) antibody
    See all N6-methyladenosine (m6a) primary antibodies
  • Description
    Rabbit polyclonal to N6-methyladenosine (m6a)
  • Tested applications
    Suitable for: Nucleotide Arraymore details
  • Species reactivity
    Reacts with: Mammal
  • Immunogen

    Chemical/ Small Molecule corresponding to N6-methyladenosine (m6a). KLH-conjugated N6-methyladenosine (m6a).

  • Positive control
    • ab151230 was tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6a) and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
  • General notes

    N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

    The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Constituent: PBS

    PBS prepared using DEPC-treated water.
    The use of RNase inhibitor is recommended.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Nucleotide Array Use a concentration of 2 µg/ml.

Target

  • Relevance
    N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
  • Alternative names
    • m6a antibody
    • N6-methyladenosine antibody

Anti-N6-methyladenosine (m6a) antibody images

  • All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6a) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

    2Ome-m6A - N6-methyladenosine

    2Ome-m6A_unmod - unmodified adenosine

References for Anti-N6-methyladenosine (m6a) antibody (ab151230)

This product has been referenced in:
  • Mauer J  et al. Reversible methylation of m(6)Am in the 5' cap controls mRNA stability. Nature 541:371-375 (2017). CLIP ; Human . Read more (PubMed: 28002401) »
  • Kan L  et al. The m(6)A pathway facilitates sex determination in Drosophila. Nat Commun 8:15737 (2017). CLIP ; Drosophila melanogaster . Read more (PubMed: 28675155) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF10A expressing Lamin B1-DAM fusion protein)
Permeabilization
No
Specification
MCF10A expressing Lamin B1-DAM fusion protein
Blocking step
FBS as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
70% Ethanol
Username

Abcam user community

Verified customer

Submitted Feb 07 2017

Application
RIP
Immuno-precipitation step
Other - Protein A Dynabeads
Detection step
Real-time PCR
Sample
Human Cell lysate - other (Purified mRNA from HeLa cell)
Specification
Purified mRNA from HeLa cell
Negative control
None.
Type
RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Positive control
A positive group using known m6A-Ab for cross-comparison.
Username

Dr. Boxuan Zhao

Verified customer

Submitted Jan 05 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BlockAid from Invitrogen as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Sample
Human Cell (Adherent cell types)
Specification
Adherent cell types
Permeabilization
Yes - PBS + 0.25% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 01 2014

The nucleotide array was performed using 2'-O-methyl-modified ribonucleotides, printed on to nitrocellulose-coated slides (the 2'-O-methyl-modification makes the oligos resistant to most ribonucleases). This array was then probed with the primary antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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