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Products:Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Overview
Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) products (20) ...
Description
Goat polyclonal to nNOS (neuronal)
Tested applications
IHC-FrFl, WB, IHC-FoFr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit
Predicted to work with
Cow, Dog, Human
Immunogen
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
C-ESKKDTDE VFSS
Positive control
WB: mouse brain lysate IHC: mouse DRG following spinal nerve ligation (2 weeks)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FrFl: Use at an assay dependent dilution.
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa).(Lyse in RIPA buffer.)
IHC-FoFr: 1/300(Ovenight incubation at room temp.)
IHC-P: Use at an assay dependent dilution. (PubMed: 16263087)
Target
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
Post-translational
modifications
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Target information above from: UniProt accessionP29475
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2310005C01Rik antibody
- bNOS antibody
- bNOS antibody
see all
Database links
- Entrez Gene: 477498 Dog
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
see all
Anti-nNOS (neuronal) antibody images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - nNOS (neuronal) antibody (ab1376)
ab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary.
Sophie Pezet, King`s college London, United Kingdom
Western blot - nNOS (neuronal) antibody (ab1376)
Predicted band size : 161 kDa
ab1376 (0.3 µg/ml) staining nNOS (neuronal) in human muscle whole tissue lysate (35 µg in RIPA). Incubation for 1 hour; detected by chemiluminescence.
References for Anti-nNOS (neuronal) antibody (ab1376)
This product has been referenced in:
- O'Malley Det al. Colonic soluble mediators from the maternal separation model of irritable bowel syndrome activate submucosal neurons via an interleukin-6-dependent mechanism. Am J Physiol Gastrointest Liver Physiol 300:G241-52 (2011). IHC-Fr; Rat.Read more (PubMed: 21109592) »
- Garzón Jet al. RGSZ2 Binds to the Neural Nitric Oxide Synthase PDZ Domain to Regulate Mu-Opioid Receptor-Mediated Potentiation of the N-Methyl-D-Aspartate Receptor-Calmodulin-Dependent Protein Kinase II Pathway. Antioxid Redox Signal 15:873-87 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21348811) »
See all 6 publications for this product
Publishing research using ab1376? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"