Anti-nNOS (neuronal) antibody (ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies -
Description
Goat polyclonal to nNOS (neuronal) -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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Positive control
- ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
Post-translational
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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Database links
- Entrez Gene: 4842 Human
- Omim: 163731 Human
- SwissProt: P29475 Human
- Unigene: 654410 Human
- Unigene: 684465 Human
- Unigene: 684466 Human
- Unigene: 684467 Human
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Alternative names
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2µg/mL). -
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (74)
ab1376 has been referenced in 74 publications.
- Buijs TJ et al. STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons. EMBO J 42:e111348 (2023). PubMed: 36524441
- Matthies M et al. Nitric oxide (NO) synthase but not NO, HNO or H2 O2 mediates endothelium-dependent relaxation of resistance arteries from patients with cardiovascular disease. Br J Pharmacol 179:1049-1064 (2022). PubMed: 34664280
- Weil T et al. Daily changes in light influence mood via inhibitory networks within the thalamic perihabenular nucleus. Sci Adv 8:eabn3567 (2022). PubMed: 35687680
- Liu Y et al. Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating H2S/CSE and NO/nNOS Systems. Evid Based Complement Alternat Med 2022:7757833 (2022). PubMed: 36091585
- Li YR et al. Involvement of nitrergic neurons in colonic motility in a rat model of ulcerative colitis. World J Gastroenterol 28:3854-3868 (2022). PubMed: 36157548