Anti-nNOS (neuronal) antibody (ab6175)
- Product nameAnti-nNOS (neuronal) antibodySee all nNOS (neuronal) primary antibodies ...
- DescriptionSheep polyclonal to nNOS (neuronal)
- SpecificityReacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
- Tested applicationsWB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Rat, Rabbit, Guinea pig
Synthetic peptide: RSESIAFIEESKKDADEVFSS conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 1409-1429 of Rat NOS1 (nNOS).
- Positive control
- rat brain
- General notesThis antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
- PurityWhole antiserum
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab6175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. It has been reported that the antibody can give mild background in some instances.|
|IHC-P||IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|IHC-Fr||IHC-Fr: 1/100 - 1/2000.|
- FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
- Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
- Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.
- DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
- Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
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Anti-nNOS (neuronal) antibody images
Rat braistem staining for nNOS with ab6175 (1:2000) (perfusion fixed frozen sections).
References for Anti-nNOS (neuronal) antibody (ab6175)
This product has been referenced in:
- Colucci M et al. Intestinal dysmotility and enteric neurochemical changes in a Parkinson's disease rat model. Auton Neurosci 169:77-86 (2012). Read more (PubMed: 22608184) »
- Buchwalow IB et al. Nitric oxide synthase in muscular dystrophies: a re-evaluation. Acta Neuropathol (Berl) 111:579-88 (2006). Read more (PubMed: 16718354) »