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Product name
nNOS (neuronal) peptide - phospho S847
See all nNOS (neuronal) products (5) ...
Protein description
Synthetic peptide derived from within residues 800 - 900 of Mouse nNOS (neuronal). (Note: the amino acid sequence is proprietary)
Expression host
CHO
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
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Western blot - nNOS (neuronal) (phospho S847) antibody (ab16650)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab16981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab16650.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
Post-translational
modifications
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Target information above from: UniProt accessionP29475
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
nNOS (neuronal) peptide - phospho S847 images:
Western blot - nNOS (neuronal) (phospho S847) antibody (ab16650)
Lanes 1 - 3 : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lane 4 : nNOS antibody at 1/2500 dilution
Lane 1 : mouse forebrain
Lane 2 : mouse forebrain with
Lane 3 : mouse forebrain with corresponding unmodified nNOS (neuronal) peptide at 1 µg/ml
Lane 4 : mouse forebrain
Lysates/proteins at 20 µg per lane.
Predicted band size : 160 kDa
Observed band size : 160 kDa
References for nNOS (neuronal) peptide - phospho S847 (ab16981)
ab16981 has not yet been referenced specifically in any publications.
Publishing research using ab16981? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"