Overview

  • Product nameAnti-nSMase antibody [EPR6718]
    See all nSMase primary antibodies
  • Description
    Rabbit monoclonal [EPR6718] to nSMase
  • Tested applicationsICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human nSMase.

  • Positive control
    • 293T, MOLT4 and K562 cell lysates; U-87MG cells; Mouse brain and Rat brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab131330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    Anti-nSMase antibody [EPR6718] images

    • All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 48 kDa
    • Immunofluorescent analysis of U-87 MG cells labelling nSMase with ab131330 at 1/250 dilution.
    • All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 48 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-nSMase antibody [EPR6718] (ab131330)

    This product has been referenced in:
    • Russ DW  et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015). WB ; Rat . Read more (PubMed: 26296420) »

    See 1 Publication for this product

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    To our knowledge, ab131330 has not been tested in Yeast. Therefore, I can offer a discount off a future purchase if you buy ab131330 now, test it in Yeast and submit feedback to us in the form...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"