Overview
- Product nameAnti-nSMase antibody [EPR6718]See all nSMase primary antibodies ...
- DescriptionRabbit monoclonal [EPR6718] to nSMase
- Tested applicationsICC/IF, WB, IP more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues in Human nSMase.
- Positive control293T, MOLT4 and K562 cell lysates; U-87MG cells; Mouse brain and Rat brain lysates.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 6.20 x 10-11 M
- Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA - PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR6718
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab131330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/250 - 1/500. |
| WB | WB: 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). |
| IP | IP: 1/10 - 1/100. |
Target
- FunctionConverts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
- Sequence similaritiesBelongs to the neutral sphingomyelinase family.
- Cellular localizationMembrane.
-
Database links
- Entrez Gene: 6610 Human
- Entrez Gene: 20598 Mouse
- Entrez Gene: 83537 Rat
- Omim: 603498 Human
- SwissProt: O60906 Human
- SwissProt: Q8C6H6 Mouse
- SwissProt: Q8BW85 Mouse
- SwissProt: O70572 Mouse
- SwissProt: Q5BKB2 Rat
- SwissProt: Q9ET64 Rat
- Unigene: 55235 Human
- Unigene: 953 Mouse
- Unigene: 18572 Rat
see all
Target information above from: UniProt accession
O60906
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ISC1 antibodyLyso platelet activating factor phospholipase C antibodyLyso-PAF-PLC antibody
- Lyso-platelet-activating factor-phospholipase C antibodyN-SMase antibodyNeutral sphingomyelinase antibodyNSMA_HUMAN antibodynSMase antibodyNSMASE1 antibodySmpd2 antibodySphingomyelin phosphodiesterase 2 antibodySphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) antibody
see all
Anti-nSMase antibody [EPR6718] images
-
![Western blot - Anti-nSMase antibody [EPR6718] (ab131330)](http://a.abcam.com/ps/datasheet/images/131/ab131330/nSMase-Primary-antibodies-ab131330-1.JPG)
Western blot - Anti-nSMase antibody [EPR6718] (ab131330)All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody [EPR6718] (ab131330)](http://a.abcam.com/ps/datasheet/images/131/ab131330/nSMase-Primary-antibodies-ab131330-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody [EPR6718] (ab131330)Immunofluorescent analysis of U-87 MG cells labelling nSMase with ab131330 at 1/250 dilution. -
![Western blot - Anti-nSMase antibody [EPR6718] (ab131330)](http://a.abcam.com/ps/datasheet/images/131/ab131330/nSMase-Primary-antibodies-ab131330-3.JPG)
Western blot - Anti-nSMase antibody [EPR6718] (ab131330)All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 48 kDa -
](http://a.abcam.com/ps/datasheet/images/131/ab131330/nSMase-Primary-antibodies-ab131330-4.jpg)
Other-Anti-nSMase antibody [EPR6718](ab131330)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-nSMase antibody [EPR6718] (ab131330)
ab131330 has not yet been referenced specifically in any publications.
