Overview

  • Product name
  • Description
    Rabbit polyclonal to NAB1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A region within synthetic peptide: ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA, corresponding to amino acids 71-120 of Human NAB1

  • Positive control
    • Human intestine, transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

ELISA: Titre using peptide based assay 1/62500.

IHC-P Use a concentration of 16 µg/ml.

Target

  • Function
    Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
  • Tissue specificity
    Isoform Short is found in a myeloid leukemia cell line.
  • Sequence similarities
    Belongs to the NAB family.
  • Domain
    The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGR 1 binding protein 1 antibody
    • EGR-1-binding protein 1 antibody
    • EGR1 binding protein 1 antibody
    • NAB1 antibody
    • NAB1_HUMAN antibody
    • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
    • NGFI A binding protein 1 antibody
    • NGFI-A-binding protein 1 antibody
    • Transcriptional regulatory protein p54 antibody
    see all

Images

  • Anti-NAB1 antibody (ab50381) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Ab50381 at 16µg/ml staining epithelial cells of human intestinal villus; paraffin embedded; magnification x400

References

ab50381 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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