Recombinant
RabMAb

Anti-NAB1 antibody [EPR8227(2)] (ab150389)

Overview

  • Product name
    Anti-NAB1 antibody [EPR8227(2)]
    See all NAB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8227(2)] to NAB1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HepG2, HeLa, U87 MG and 293T cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab150389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
    • Tissue specificity
      Isoform Short is found in a myeloid leukemia cell line.
    • Sequence similarities
      Belongs to the NAB family.
    • Domain
      The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
      The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EGR 1 binding protein 1 antibody
      • EGR-1-binding protein 1 antibody
      • EGR1 binding protein 1 antibody
      • NAB1 antibody
      • NAB1_HUMAN antibody
      • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
      • NGFI A binding protein 1 antibody
      • NGFI-A-binding protein 1 antibody
      • Transcriptional regulatory protein p54 antibody
      see all

    Images

    • All lanes : Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 54 kDa

    References

    ab150389 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Francis Mcmanus

    Verified customer

    Submitted Apr 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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