Anti-Nab2 antibody [cl.1C4] (ab11987)

Overview

  • Product nameAnti-Nab2 antibody [cl.1C4]
    See all Nab2 primary antibodies
  • Description
    Mouse monoclonal [cl.1C4] to Nab2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

  • Positive control
    • K-562 nuclear extract (TPA stimulated).

Properties

Applications

Our Abpromise guarantee covers the use of ab11987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 56-65 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionActs as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • Tissue specificityWidely expressed at low levels. Highly expressed in melanoma cell lines.
  • Sequence similaritiesBelongs to the NAB family.
  • DomainThe NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • Cellular localizationNucleus. Isoform 2 is not localized to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

Anti-Nab2 antibody [cl.1C4] images

  • Detection of Nab2 by Western blot analysis. Antibody experiment using Nab2 mouse monoclonal antibody demonstrates the presence of Nab2 transcription factor in nuclear extracts derived from K-562 cells (TPA) using a 1/1000 dilution

References for Anti-Nab2 antibody [cl.1C4] (ab11987)

ab11987 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Here is the information that I found about how to make this lysate: Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting....

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