Overview

  • Product nameAnti-NACA1 antibody
  • Description
    Mouse polyclonal to NACA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length NACA1 protein (Human)

  • Positive control
    • NACA1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 23 kDa).

Target

  • FunctionPrevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the NAC-alpha family.
    Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    Contains 1 UBA domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Also found in nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha NAC antibody
    • Alpha-NAC antibody
    • Hom s 2.02 antibody
    • HSD48 antibody
    • MGC117224 antibody
    • NAC-alpha antibody
    • naca antibody
    • NACA/1.9.2 antibody
    • NACA_HUMAN antibody
    • NACA1 antibody
    • Nascent polypeptide associated complex alpha polypeptide antibody
    • Nascent polypeptide associated complex alpha subunit antibody
    • Nascent polypeptide associated complex subunit alpha antibody
    • Nascent polypeptide-associated complex subunit alpha antibody
    see all

Anti-NACA1 antibody images

  • All lanes : Anti-NACA1 antibody (ab67872) at 1/500 dilution

    Lane 1 : NACA1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-NACA1 antibody (ab67872)

ab67872 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra, pH 6.0
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"