Overview

  • Product nameAnti-NACA2 antibody
  • Description
    Mouse polyclonal to NACA2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NACA2 (NP_954984.1).

  • Positive control
    • NACA2 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa).

Target

  • RelevanceNACA2, Nascent polypeptide associated complex subunit alpha 2, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER) by binding to nascent polypeptide chains as they emerge from the ribosome and blocking their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA2 also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Alpha NAC like antibody
    • ANAC antibody
    • Hom s 2.01 antibody
    • MGC71999 antibody
    • NAC alpha like antibody
    • NACA2 antibody
    • NACAL antibody
    • nascent polypeptide associated complex alpha polypeptide like antibody
    • Nascent polypeptide associated complex subunit alpha 2 antibody
    see all

Anti-NACA2 antibody images

  • All lanes : Anti-NACA2 antibody (ab69439) at 1/500 dilution

    Lane 1 : NACA2 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-NACA2 antibody (ab69439)

ab69439 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic fibroblast)
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Loading amount 30 µg
Specification Embryonic fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

YanPing Zhu

Verified customer

Submitted Jul 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"