Biological descriptionPotent endogenous cannabinoid and vanilloid receptor agonist. (Ki values are 0.25 and 12 μM for CB1 and CB2, respectively). Potent agonist at TRPV1 (VR1) receptors (EC50 = ~50 nM). Active in vivo.
Storage instructionsStore at -20°C (desiccating conditions).
Solubility overviewSupplied in ethanol (5 mg/ml)
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab15580 staining Ki67 in SK-N-SH cells treated with NADA (N-Arachidonyldopamine) (ab120099), by ICC/IF. Decrease in Ki67 expression correlates with increased concentration of NADA (N-Arachidonyldopamine), as described in literature. The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120099 (NADA (N-Arachidonyldopamine)) in ethanol, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15580 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for NADA (N-Arachidonyldopamine) (ab120099)
This product has been referenced in:
O'Sullivan SE et al. Characterisation of the vasorelaxant properties of the novel endocannabinoid N-arachidonoyl-dopamine (NADA). Br J Pharmacol141:803-12 (2004).
Read more (PubMed: 14769783) »
Huang SM et al. An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. Proc Natl Acad Sci U S A99:8400-5 (2002).
Read more (PubMed: 12060783) »
Bisogno T et al. N-acyl-dopamines: novel synthetic CB(1) cannabinoid-receptor ligands and inhibitors of anandamide inactivation with cannabimimetic activity in vitro and in vivo. Biochem J351 Pt 3:817-24 (2000).
Read more (PubMed: 11042139) »