Overview

  • Product nameAnti-NADE antibody
    See all NADE primary antibodies
  • Description
    Rabbit polyclonal to NADE
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the middle of NADE (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab21303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 13 kDa).

Target

  • FunctionMay be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.
  • Tissue specificityFound in ovarian granulosa cells, testis, prostate and seminal vesicle tissue. High levels also detected in liver.
  • Sequence similaritiesBelongs to the BEX family.
  • DomainThe nuclear export signal is required for export from the nucleus and the interactions with itself and p75NTR/NGFR.
  • Post-translational
    modifications
    Ubiquitinated. Degraded by the proteasome.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the cytoplasm and the nucleus. Associates with replicating mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bex antibody
    • BEX3 antibody
    • BEX3_HUMAN antibody
    • Brain expressed X linked 3 (mouse) homolog antibody
    • brain expressed, X-linked 3 antibody
    • Brain-expressed X-linked gene 3 antibody
    • Brain-expressed X-linked protein 3 antibody
    • DXS6984E antibody
    • HGR74 antibody
    • Nerve growth factor receptor (TNFRSF16) associated protein 1 antibody
    • Nerve growth factor receptor associated protein 1 antibody
    • Nerve growth factor receptor-associated protein 1 antibody
    • NGFR-associated protein 1 antibody
    • NGFRAP1 antibody
    • Ovarian granulosa cell 13.0 kDa protein HGR74 antibody
    • ovarian granulosa cell protein (13kD) antibody
    • Ovarian granulosa cell protein antibody
    • p75NTR associated cell death executor antibody
    • p75NTR-associated cell death executor antibody
    • Protein BEX3 antibody
    see all

Anti-NADE antibody images

  • All lanes : Anti-NADE antibody (ab21303) at 1 µg/ml

    Lane 1 : human brain cell lysate with blocking peptide.
    Lane 2 : human brain cell lysate with Without blocking peptide.


    Predicted band size : 13 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  • ab21303 at 2µg/ml staining NADE in human brain tissue by IHC 

References for Anti-NADE antibody (ab21303)

This product has been referenced in:
  • Xiao L & Tsutsui T Three-dimensional epithelial and mesenchymal cell co-cultures form early tooth epithelium invagination-like structures: Expression patterns of relevant molecules. J Cell Biochem : (2012). Read more (PubMed: 22234822) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount 30 µg
Specification Small Cell Lung Cancer
Gel Running Conditions Reduced Denaturing (15% Gel)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"