Anti-NADH dehydrogenase subunit 4 antibody [9E4-2D8] - N-terminal (ab219822)

Overview

  • Product name
    Anti-NADH dehydrogenase subunit 4 antibody [9E4-2D8] - N-terminal
  • Description
    Mouse monoclonal [9E4-2D8] to NADH dehydrogenase subunit 4 - N-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NADH dehydrogenase subunit 4 (N terminal).
    Database link: P03905

  • Positive control
    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).

Properties

Applications

Our Abpromise guarantee covers the use of ab219822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).

Western blot using whole cell extracts is not recommended.

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in MT-ND4 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-ND4 are a cause of Leber hereditary optic neuropathy with dystonia (LDYT) [MIM:500001]; also called familial dystonia with visual failure and striatal lucencies. LDYT is part of a spectrum of Leber hereditary optic neuropathy. It is characterized by the association of optic atrophy and central vision loss with dystonia.
    Defects in MT-ND4 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
  • Sequence similarities
    Belongs to the complex I subunit 4 family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MT-ND4 antibody
    • MTND4 antibody
    • NADH dehydrogenase subunit 4 antibody
    • NADH-ubiquinone oxidoreductase chain 4 antibody
    • NADH4 antibody
    • ND4 antibody
    • NU4M_HUMAN antibody
    see all

Images

  • All lanes : Anti-NADH dehydrogenase subunit 4 antibody [9E4-2D8] - N-terminal (ab219822) at 4 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References

ab219822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219822.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up