Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821)

Overview

  • Product name
    Anti-NADH2 antibody [9E12-1B3] - N-terminal
    See all NADH2 primary antibodies
  • Description
    Mouse monoclonal [9E12-1B3] to NADH2 - N-terminal
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to Human NADH2 (N terminal).
    Database link: P03891

  • Positive control
    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).

Properties

Applications

Our Abpromise guarantee covers the use of ab219821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).

Western blot using whole cell extracts is not recommended.

Target

  • Relevance
    NADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
  • Cellular localization
    Mitochondrion inner membrane; Multi-pass membrane protein.
  • Alternative names
    • MTND2 antibody
    • NADH dehydrogenase subunit 2 antibody
    • NADH ubiquinone oxidoreductase chain 2 antibody
    • ND2 antibody

Anti-NADH2 antibody [9E12-1B3] - N-terminal images

  • All lanes : Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References for Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821)

ab219821 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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