The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).
Western blot using whole cell extracts is not recommended.
NADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Western blot - Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821)
All lanes : Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn) Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 39 kDa Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
has not yet been referenced specifically in any publications.
Publishing research using ab219821? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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