The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
Belongs to the NAF1 family.
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells
Immunofluorescent staining of human cell line U-2 OS (Fixation/Permeabilization: PFA/Triton X-100)
with ab122323 at 4ug/ml shows positivity in cytoplasm, centrosome & cell junctions.
Antibody staining is shown in green.