Overview

  • Product name
  • Description
    Rabbit polyclonal to NAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 50-100 of Human NAF1 (NP_612395.2).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 54 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
  • Sequence similarities
    Belongs to the NAF1 family.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
    • hNAF1 antibody
    • Naf1 antibody
    • NAF1_HUMAN antibody
    • Nuclear assembly factor 1 homolog antibody
    • Nuclear assembly factor 1 ribonucleoprotein antibody
    see all

Images

  • All lanes : Anti-NAF1 antibody (ab157106) at 1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 30 seconds
  • Detection of NAF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157106 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157106 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:
  • Stanley SE  et al. Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema. Sci Transl Med 8:351ra107 (2016). Read more (PubMed: 27510903) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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