Recombinant
RabMAb

Anti-NAF1 antibody [EPR11516] (ab171078)

Overview

  • Product name
    Anti-NAF1 antibody [EPR11516]
    See all NAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11516] to NAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAF1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96HR8

  • Positive control
    • HeLa, A431, A549, Namalwa and Caco-2 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
    • Sequence similarities
      Belongs to the NAF1 family.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
      • hNAF1 antibody
      • Naf1 antibody
      • NAF1_HUMAN antibody
      • Nuclear assembly factor 1 homolog antibody
      • Nuclear assembly factor 1 ribonucleoprotein antibody
      see all

    Images

    • All lanes : Anti-NAF1 antibody [EPR11516] (ab171078) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Namalwa cell lysate
      Lane 5 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling NAF1 with ab171078 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

    References

    ab171078 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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