Overview

  • Product name
  • Description
    Rabbit polyclonal to NAGLU
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 252-487 of Human NAGLU (UniProt: P54802).

  • Positive control
    • Raji whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab154508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function
    Involved in the degradation of heparan sulfate.
  • Tissue specificity
    Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
  • Involvement in disease
    Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha N acetylglucosaminidase antibody
    • alpha N acetylglucosaminidase, lysosomal antibody
    • Alpha-N-acetylglucosaminidase 77 kDa form antibody
    • ANAG antibody
    • ANAG_HUMAN antibody
    • CMT2V antibody
    • MPS IIIB antibody
    • MPS3B antibody
    • N acetyl alpha glucosaminidase antibody
    • N acetylglucosaminidase, alpha antibody
    • N-acetyl-alpha-glucosaminidase antibody
    • NAG antibody
    • NAGLU antibody
    • UFHSD 1 antibody
    • UFHSD antibody
    • UFHSD1 antibody
    see all

Images

  • Anti-NAGLU antibody (ab154508) at 1/1000 dilution + Raji whole cell
    lysate at 30 µg

    Predicted band size: 82 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells (4% at RT, 15mins) labeling NAGLU with ab154508 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling NAGLU with ab154508 at 1/500 dilution.

References

ab154508 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154508.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up