Overview

  • Product name
  • Description
    Rabbit polyclonal to NAGLU
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAGLU.

    (Peptide available as ab86400.)

  • Positive control
    • This antibody gave a positive signal in HepG2 Whole Cell Lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Target

  • Function
    Involved in the degradation of heparan sulfate.
  • Tissue specificity
    Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
  • Involvement in disease
    Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha N acetylglucosaminidase antibody
    • alpha N acetylglucosaminidase, lysosomal antibody
    • Alpha-N-acetylglucosaminidase 77 kDa form antibody
    • ANAG antibody
    • ANAG_HUMAN antibody
    • CMT2V antibody
    • MPS IIIB antibody
    • MPS3B antibody
    • N acetyl alpha glucosaminidase antibody
    • N acetylglucosaminidase, alpha antibody
    • N-acetyl-alpha-glucosaminidase antibody
    • NAG antibody
    • NAGLU antibody
    • UFHSD 1 antibody
    • UFHSD antibody
    • UFHSD1 antibody
    see all

Images

  • Anti-NAGLU antibody (ab72178) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ICC/IF image of ab72178 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5ug/ml and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

References

This product has been referenced in:
  • Kan SH  et al. Delivery of an enzyme-IGFII fusion protein to the mouse brain is therapeutic for mucopolysaccharidosis type IIIB. Proc Natl Acad Sci U S A 111:14870-5 (2014). Mouse . Read more (PubMed: 25267636) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Lec2)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
Lec2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Standard
Permeabilization
No
Blocking step
S Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 11 2012

The rabbits used for recent lots are Oryctolagus cuniculus - Sandy Half-Lop.



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Jurkat Ms Brain Ms Liver MEF F9)
Loading amount
20 µg
Specification
Jurkat Ms Brain Ms Liver MEF F9
Gel Running Conditions
Reduced Denaturing (Pierce Precise Protein Gel 8-16%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Feb 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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