Overview

  • Product name
    Anti-NAGS antibody
  • Description
    Rabbit polyclonal to NAGS
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: PVLGGTPYLD KFVVSSSRQG QGSGQMLWEC LRRDLQTLFW RSRVTNPINP, corresponding to C terminal amino acids 434-483 of Human NAGS

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab65536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Plays a role in the regulation of ureagenesis by producing variable amounts of N-acetylglutamate (NAG), thus modulating carbamoylphosphate synthase I (CPSI) activity.
  • Tissue specificity
    Highly expressed in the adult liver, kidney and small intestine. Weakly expressed in the fetal liver, lung, pancreas, placenta, heart and brain tissue.
  • Pathway
    Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.
  • Involvement in disease
    N-acetylglutamate synthase deficiency
  • Sequence similarities
    Belongs to the acetyltransferase family.
    Contains 1 N-acetyltransferase domain.
  • Post-translational
    modifications
    Probably processed by mitochondrial processing peptidase (MPP). The long form has not yet been isolated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700120E20Rik antibody
    • AGAS antibody
    • AI415708 antibody
    • Amino-acid acetyltransferase antibody
    • ARGA antibody
    • EC 2.3.1.1 antibody
    • MGC133025 antibody
    • N-acetylglutamate synthase antibody
    • N-acetylglutamate synthase conserved domain form antibody
    • N-acetylglutamate synthase, mitochondrial antibody
    • Nags antibody
    • NAGS_HUMAN antibody
    • RP23-398F7.13 antibody
    see all

Images

  • Anti-NAGS antibody (ab65536) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References

ab65536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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