Overview

  • Product name
  • Description
    Rabbit polyclonal to NAGS
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: PVLGGTPYLD KFVVSSSRQG QGSGQMLWEC LRRDLQTLFW RSRVTNPINP, corresponding to C terminal amino acids 434-483 of Human NAGS

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab65536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Plays a role in the regulation of ureagenesis by producing variable amounts of N-acetylglutamate (NAG), thus modulating carbamoylphosphate synthase I (CPSI) activity.
  • Tissue specificity
    Highly expressed in the adult liver, kidney and small intestine. Weakly expressed in the fetal liver, lung, pancreas, placenta, heart and brain tissue.
  • Pathway
    Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.
  • Involvement in disease
    N-acetylglutamate synthase deficiency
  • Sequence similarities
    Belongs to the acetyltransferase family.
    Contains 1 N-acetyltransferase domain.
  • Post-translational
    modifications
    Probably processed by mitochondrial processing peptidase (MPP). The long form has not yet been isolated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700120E20Rik antibody
    • AGAS antibody
    • AI415708 antibody
    • Amino-acid acetyltransferase antibody
    • ARGA antibody
    • EC 2.3.1.1 antibody
    • MGC133025 antibody
    • N-acetylglutamate synthase antibody
    • N-acetylglutamate synthase conserved domain form antibody
    • N-acetylglutamate synthase, mitochondrial antibody
    • Nags antibody
    • NAGS_HUMAN antibody
    • RP23-398F7.13 antibody
    see all

Anti-NAGS antibody images

  • Anti-NAGS antibody (ab65536) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-NAGS antibody (ab65536)

ab65536 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65536.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up