Overview

  • Product name
  • Description
    Rabbit polyclonal to NAIP
  • Specificity
    This antibody detects recombinant human NAIP in extracts from Sf21 insect cells, but it is unknown as to whether this antibody detects the endogenous form.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide surrounding amino acid 506 of human NAIP.

  • General notes


    NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa.

Target

  • Function
    Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
  • Tissue specificity
    Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
  • Sequence similarities
    Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Baculoviral IAP repeat containing 1 antibody
    • Baculoviral IAP repeat-containing protein 1 antibody
    • BIRC 1 antibody
    • BIRC1 antibody
    • BIRC1_HUMAN antibody
    • Birc1a antibody
    • FLJ42520 antibody
    • NAIP antibody
    • Naip1 antibody
    • Neuronal apoptosis inhibitory protein antibody
    • NLR family apoptosis inhibitory protein antibody
    • NLR family BIR domain containing 1 antibody
    • NLRB 1 antibody
    • NLRB1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
    • Psi neuronal apoptosis inhibitory protein antibody
    • psiNAIP antibody
    • Similar to occludin antibody
    see all

Anti-NAIP antibody images

  • All lanes : Anti-NAIP antibody (ab2549) at 1/1000 dilution

    Lane 1 : Lysate prepared from transfected PC3 cells.
    Lane 2 : Lysate prepared from non-transfected PC3 cells.
    Lane 3 : Lysate prepared from transfected HeLa cells.
    Lane 4 : Lysate prepared from non-transfected HeLa cells.
    Lane 5 : Lysate prepared from non-transfected NTera2D1 cells.

    Secondary
    secondary antibody at 1/100000 dilution

    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa (possible isoform),115 kDa,130 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Image from Dr MT Romanish et al, PLoS One. 2009 Jun 2;4(6):e5761, Fig 5.

References for Anti-NAIP antibody (ab2549)

This product has been referenced in:
  • Romanish MT  et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB ; Human . Read more (PubMed: 19488400) »

See 1 Publication for this product

Product Wall

a.a. 473-490 of human NAIP.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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