Overview

  • Product nameAnti-NAK/TBK1 antibody [108A429]
    See all NAK/TBK1 primary antibodies
  • Description
    Mouse monoclonal [108A429] to NAK/TBK1
  • Specificityab12116 recognises human NAK.
  • Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human NAK/TBK1 .

  • Positive control
    • 293 cell extracts transfected with human TBK1 cDNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab12116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa).
IP Use at an assay dependent concentration.

Target

  • FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
  • Tissue specificityUbiquitous with higher expression in testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • FLJ11330 antibody
    • NAK antibody
    • NF kappa B activating kinase antibody
    • NF kB activating kinase antibody
    • NF-kappa-B-activating kinase antibody
    • Serine/threonine protein kinase TBK 1 antibody
    • Serine/threonine protein kinase TBK1 antibody
    • Serine/threonine-protein kinase TBK1 antibody
    • T2K antibody
    • TANK binding kinase 1 antibody
    • TANK-binding kinase 1 antibody
    • TBK 1 antibody
    • Tbk1 antibody
    • TBK1_HUMAN antibody
    see all

Anti-NAK/TBK1 antibody [108A429] images



  • Predicted band size : 87.5 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NAK knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab12116 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab12116 was shown to recognize NAK when NAK knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK knockout samples were subjected to SDS-PAGE. ab12116 and ab181602 (loading control to GAPDH) were diluted at 1 μg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-NAK/TBK1 antibody [108A429] (ab12116) at 2 µg/ml

    Lane 1 : 293 cells
    Lane 2 : 293 cells transfected with Human NAK cDNA


    Predicted band size : 87.5 kDa
  • Overlay histogram showing HeLa cells stained with ab12116 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12116, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-NAK/TBK1 antibody [108A429] (ab12116)

This product has been referenced in:
  • Charoenthongtrakul S  et al. RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. PLoS One 8:e53717 (2013). WB . Read more (PubMed: 23308279) »
  • Gao L  et al. ABIN1 Protein Cooperates with TAX1BP1 and A20 Proteins to Inhibit Antiviral Signaling. J Biol Chem 286:36592-602 (2011). WB, IP . Read more (PubMed: 21885437) »

See all 3 Publications for this product

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