• Product nameAnti-NAK/TBK1 antibody [EP611Y]
    See all NAK/TBK1 primary antibodies
  • Description
    Rabbit monoclonal [EP611Y] to NAK/TBK1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NAK/TBK1 aa 1-100 (N terminal).

  • Positive control
    • Hela cells, human breast carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab40676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
IHC-P 1/50.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
    • Tissue specificityUbiquitous with higher expression in testis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC antibody
      • FLJ11330 antibody
      • NAK antibody
      • NF kappa B activating kinase antibody
      • NF kB activating kinase antibody
      • NF-kappa-B-activating kinase antibody
      • Serine/threonine protein kinase TBK 1 antibody
      • Serine/threonine protein kinase TBK1 antibody
      • Serine/threonine-protein kinase TBK1 antibody
      • T2K antibody
      • TANK binding kinase 1 antibody
      • TANK-binding kinase 1 antibody
      • TBK 1 antibody
      • Tbk1 antibody
      • TBK1_HUMAN antibody
      see all

    Anti-NAK/TBK1 antibody [EP611Y] images

    • Predicted band size : 84 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: NAK knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab40676 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab40676 was shown to specifically react with NAK when NAK knockout samples were used. Wild-type and NAK knockout samples were subjected to SDS-PAGE. ab40676 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000  respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab40676 diluted 1:50.
    • Immunofluorescent staining of HeLa cells using ab40676 diluted 1:500.
    • Anti-NAK/TBK1 antibody [EP611Y] (ab40676) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Predicted band size : 84 kDa
      Observed band size : 84 kDa

    References for Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

    This product has been referenced in:
    • Sirohi K  et al. A Glaucoma-Associated Variant of Optineurin, M98K, Activates Tbk1 to Enhance Autophagosome Formation and Retinal Cell Death Dependent on Ser177 Phosphorylation of Optineurin. PLoS One 10:e0138289 (2015). Read more (PubMed: 26376340) »
    • Abe T & Barber GN Cytosolic-DNA-mediated, STING-dependent proinflammatory gene induction necessitates canonical NF-?B activation through TBK1. J Virol 88:5328-41 (2014). Read more (PubMed: 24600004) »

    See all 10 Publications for this product

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